Liquid Media

Ready-to-use media considerably reduce the preparation time in quality control laboratories and also effectively reduce the risks of cross contamination. GVS is cooperating closely with quality assurance managers in the industry in the development of its own media and test kits.

This intensive product development has produced a range of products that is being used to monitor production plants and conduct microbiological checks on raw materials through to final product release in laboratories in more than 40 countries.

Features and benefits

• Wide range of products satisfies even special customer requirements
• Optimal media stability, sterility, and reproducibility
• Less time-consuming, higher productivity
• Batch-specific quality certificate in each pack 

Applications

• Microbiological analysis of:
• Drinking water
• Surface water
• Recreational water
• Purified water
• Media Descriptions

Liquid Media Description

Brilliant Green Bile Broth 2% 

BGBB contains two inhibitors of both gram-positive and selected gram-negative organisms, namely, oxgall and brilliant green dye. Fermentation is detected by gas production.

Cetrimide Broth

Pseudomonas aeruginosa is characterized by the production of pyocyanin (a blue green, water soluble, non-fluorescent, phenazine pigment) which is stimulated by the inclusion of magnesium chloride and potassium sulfate in the broth. Cetrimide (N-cetyl-NNN-trimethylammonium bromide) is added to inhibit bacteria other than Pseudomonas aeruginosa. Its action as a quaternary ammonium cationic detergent causes nitrogen and phosphorous to be released from bacterial cells other than Pseudomonas aeruginosa. 

ColiCeck with MUG

ColiCheck with MUG is used for presumptive identification of coliforms and the determination of the presence of E.coli in water samples by a presence/absence method.

EC Broth

EC Broth contains casein peptone as a source of nutrients. Lactose provides the carbohydrate fermented by coliform bacteria and Escherichia coli. In addition, lactose-positive bacteria metabolize lactose with gas formation. Gram-positive bacteria are inhibited by the mixture of bile salts.

EC Broth with MUG

The presence of fluorescence using a long-wave UV light source confirms the presence of Escherichia coli and no further confirmation is required. MUG detects anaerogenic strains, which may not be detected in the conventional procedure. Lactose is a source of energy. Casein peptone provides additional nutrients. The mixture of bile salts is inhibiting for gram-positive bacteria, particularly bacilli and fecal streptococci. The substrate 4-methylumbelliferyl-b-D-glucuronide is hydrolyzed by an enzyme, b-glucuronidase, possessed by most Escherichia coli and a few strains of Salmonella, Shigella and Yersinia, to produce a fluorescent end product, 4-methylumbelliferone.

Enterococcus Broth

Enterococcus Broth is a modified version of the improved media described by Slanetz and Bartley with triphenyltetrazolium chloride (TTC). The membrane filtration method is simple to perform, does not require confirmation and permits a direct count of enterococci in 48 hours.

Heterotrophic Plate Count (HPC) Broth with or without TTC

HPC Broth and HPC Broth with TTC Heterotrophic Plate Count (HPC) Broth is used to determine live heterotrophs in drinking water and other media at incubation temperatures of 35°C. All bacteria grow on HPC with indicator media and produce a red color. This is a result of the precipitation of formazan following the reduction of 2,3,5- TTC by bacteria.

KF-Streptococcus Broth

KF-Streptococcus Broth is selective for the determination of fecal streptococci in polluted surface waters. Maltose and lactose are fermentable carbohydrates, sodium azide is the selective agent and bromcresol purple is the indicator dye.

Lauryl Sulfate or Lauryl Tryptose Broth

This media was developed for the detection of coliform organisms by the American Public Health Association (APHA). It is now the standard medium of choice in the presumptive phase of the standard coliform MPN test for the microbiological examination of water. 

Mannitol Salt Broth

Because of the amount of peptones and beef extract, Mannitol Salt is a nutrient rich medium. Most bacteria (other than staphylococci) are inhibited by the high concentration of sodium chloride. Organisms capable of fermenting mannitol, e.g., Staphylococcus aureus, cause a pH change in the media. With phenol red as the pH indicator the colonies appear with a yellow coloration.

Membrane Lauryl Sulfate Broth

This media was developed for the detection of coliform organisms and is now the media of choice for the enumeration of total coliforms and Escherichia coli in the United Kingdom. This media replaced membrane enriched broth containing 0.4% Teepol 610.

M-Endo Coliform Broth

M-Endo is a red colored media, which needs to be stored in the dark to prevent discoloration. Gram-positive bacteria are inhibited on this media by the desoxycholate and lauryl sulfate. The addition of ethanol increases the antibacterial nature of the formulation. Lactose fermenting organisms form aldehydes, which react with Schiff’s reagent (basic fuchsin and sodium sulfite) to give red colored zones around the colonies. Coliform colonies are therefore red with a characteristic metallic sheen.

M-FC Broth

Allows the development of fecal coliforms at elevated temperatures (44.5°C).

M-FC with Rosolic Acid

M-FC with Rosolic Acid acts and functions in the same way as M-FC Broth. Rosolic acid inhibits bacterial growth in general, except for fecal coliforms.

M-Green Yeast and Mold Broth

M-Green Yeast and Mold Broth is an improved modification of the liquid media. The addition of bromocresol green, which diffuses into fungal colonies as an alkaline reaction, allows them to be easily identified. Metabolic by-products from the developing colonies diffuse into the surrounding medium, further reducing the pH which aids in the inhibition of bacterial growth, but also produces an acid reaction that causes residual bromocresol green to change to yellow.

M-Green Select Broth

M-Green Select Broth was developed to improve efficiency of detection and enumeration of fungi in sugar based drinks using the membrane filtration method. This medium has a low pH, which inhibits bacterial growth to allow for the development and enumeration of yeast and mold.

MI Broth and MI Agar

MI Broth detects the presence of coliform bacteria by the production of b-galactosidase, which cleaves the substrate MUGal to produce 4-methylumbelliferone, which fluoresces on exposure to UV light. Non-coliforms do not produce this enzyme and therefore do not fluoresce on the medium. Escherichia coli is detected by the compound IBDG. The b-glucuronidase produced by Escherichia coli cleaves the substrate to produce a blue indigo color in the colonies. As Escherichia coli is also a total coliform, and also produces b-galactosidase, it will also fluoresce. The antibiotic cefsulodin is present to inhibit the growth of gram-positive bacteria and some non-coliform gram-negative bacteria that can cause false positive reactions.

MRS Broth

MRS medium supports luxuriant growth of all lactobacilli, even the slow growing species.

M-TGE Total Count Media

All bacteria develop on TGE media and produce a range of different colored and sized colonies.

Orange Serum Media

Organisms known to grow in single strength and concentrated juices are lactic acid and acetic acid bacteria and yeast. Lactobacilli, Leuconostoc and yeast have all been identified as spoilage organisms by numerous authors. Orange serum at pH 5.4 to 5.6 has been reported to yield maximum counts of all types of spoilage organisms in mixed cultures and in single culture comparison tests.

Potato Dextrose Broth and Agar Media

Potato Dextrose Broth is recommended in Standard Methods as the media that gives the most consistent and highest counts for the recoveries of yeast and mold in dairy products. The inclusion of potato extract encourages the growth and development of fungi. Sterile tartaric acid may be added to lower the pH to 3.5 ± 0.2 to further inhibit the growth of conflicting bacteria. 

PRY Broth

Preservative Resistant yeast Broth is a low ph selective medium for the detection of spoilage microorganism in beverages and water.

Pseudomonas Broth

Pseudomonas aeruginosa is characterized by the production of pyocyanin (a blue green, water soluble, non-fluorescent, phenazine pigment) which is stimulated by the inclusion of magnesium chloride and potassium sulfate in the broth. Irgasan, an antimicrobial agent, selectively inhibits gram-positive and gram-negative bacteria other than pseudomonads. Glycerol both serves as an energy source and helps in the promotion of pyocyanin.

Sabouraud Dextrose Broth

Peptone in the media is used as a nitrogen source for the development of fungi. Dextrose acts as an energy source for the growth of microorganisms. The low pH is favorable for the development of fungi, especially dermatophytes, but at the same time inhibits the development of contaminating bacteria from clinical specimens.

Standard Methods Agar

All bacteria develop on Standard Methods and produce a range of different colored and sized colonies.

Total Count Media with TTC

All bacteria develop on Total Count Media with indicator and produce a red color as a result of the precipitation of formazan following the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) by bacteria.

Trypticase Soy Broth – Single Strength

General purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms. Trypticase Soy Broth – Single Strength complies with the demands of the DIN Norm 10167 for the detection of Escherichia coli serotype 0157:H7 in foods and FDA-BAM for the isolation of enterohemorrhagic Escherichia coli (EHEC). In addition the media conforms to the formula of the US Pharmacopoeia.

Trypticase Soy Broth – Double Strength

TSB is a medium that will support the growth of a wide variety of microorganisms including aerobic, facultative, and anaerobic bacteria and fungi.

Wallerstein Nutrient Broth (WL) and WL Differential Broth (WLD)

WL Nutrient Broth is for the cultivation and enumeration of yeast and WL Differential Broth is for determination of bacterial count. Use of the medium at pH 5.5 and incubation at 25°C will give reliable counts for brewer’s yeast. Adjustment of the pH to 6.5 and incubation at 30°C allows for the selective growth of baker’s and distiller’s yeast